diff --git a/tests/test_with_catch/test_subinterpreter.cpp b/tests/test_with_catch/test_subinterpreter.cpp
index 3c7c35be1..8f30c8f44 100644
--- a/tests/test_with_catch/test_subinterpreter.cpp
+++ b/tests/test_with_catch/test_subinterpreter.cpp
@@ -90,7 +90,11 @@ TEST_CASE("Single Subinterpreter") {
     unsafe_reset_internals_for_single_interpreter();
 }
 
-#    if PY_VERSION_HEX >= 0x030D0000
+// "Move Subinterpreter" test is disabled on free-threaded Python 3.14+ due to a hang
+// in Py_EndInterpreter() when the subinterpreter is destroyed from a different thread
+// than it was created on. See: https://github.com/pybind/pybind11/pull/5933
+#    if PY_VERSION_HEX >= 0x030D0000                                                               \
+        && !(PY_VERSION_HEX >= 0x030E0000 && defined(Py_GIL_DISABLED))
 TEST_CASE("Move Subinterpreter") {
     std::unique_ptr<py::subinterpreter> sub(new py::subinterpreter(py::subinterpreter::create()));